IIS 6 domain gives 404 on sp2 and .NET35 update

hey all i have 3 domains in IIS6 and after update to sp2 on win2003 the site gives me a "page not found 404"

the other two work fine so it not server wide !! and it s NOT port 80

how can i troubleshoot this for the cause?

edit: its the default website from first install thats affected and its using non-port80

Welcome to Devshed!

Please correct my assumptions if they are wrong...

I assume you mean when you enter the URL into your browser it immediately gives you the 404. Is this happening from a workstation? The internet? Does it happen when you try to access the page from the server itself? With multiple domains, I am guessing you are using host headers...

A little more general background would also help:
1) Is this a stand-alone server (in or out of a DMZ) or joined to a domain?
2) Is this a public website or a web interface for an application (such as a web interface for a client/server anti-virus program)

A couple of things I would check:
1) Default page: Right click your Default Website and go to properties. When the properties dialog box opens, click the 'Documents' tab. The 'Enable default content page' checkbox should be checked. This, as you know, tells IIS which page to serve up first when a request to your server is made. Is your default page listed there?
2) Home Directory: Click the 'Home Directory' tab to make sure it is pointing to the correct folder or path.
3) DNS: Does this happen everywhere? The internet, directly on the server itself, from a workstation on your LAN?
4) If you simply click 'websites' in IIS manager on the left, does it show 'running' (in the pane to the right) next to 'Default website'?

i have tried to connect from the server thru localhost and from LAN

i dont think i have changed anything apart from updating to sp2 and install .NET3.5.

the other 2 sites (NOT subfolders) are working